Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation
Identifieur interne : 004136 ( Main/Exploration ); précédent : 004135; suivant : 004137Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation
Auteurs : Christopher Chang [États-Unis] ; Russell C. Hirsch [États-Unis] ; Don Ganem [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: The pregenomic RNA of hepadnaviruses serves as both the mRNA for the core and polymerase proteins and the RNA template for reverse transcription. We have identified a region in the duck hepatitis B virus pregenomic RNA transcription unit that is critical for the accumulation of this transcript. This 85-nt region, termed α, is located within the preC region; deletion of α results in drastically reduced steady-state levels of pregenomic RNA. This effect is not due to reduction in transcription initiation or to enhancement of premature polyadenylation at the 5′ copy of the viral poly(A) signal. However, this phenotype is suppressed by deletion of a second, larger region (β) located ca. 1 kb downstream. The activity of the α element is tissue- and species-nonspecific; however, it displays absolute orientation-dependence and its activity is influenced by its position within the transcript. Models for its action are discussed.
Url:
DOI: 10.1006/viro.1995.1115
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001038
- to stream Istex, to step Curation: 001038
- to stream Istex, to step Checkpoint: 001827
- to stream Main, to step Merge: 004197
- to stream Main, to step Curation: 004136
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation</title><author><name sortKey="Chang, Christopher" sort="Chang, Christopher" uniqKey="Chang C" first="Christopher" last="Chang">Christopher Chang</name></author><author><name sortKey="Hirsch, Russell C" sort="Hirsch, Russell C" uniqKey="Hirsch R" first="Russell C." last="Hirsch">Russell C. Hirsch</name></author><author><name sortKey="Ganem, Don" sort="Ganem, Don" uniqKey="Ganem D" first="Don" last="Ganem">Don Ganem</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A4ECD776048990F51F3B984A5224A65AB2CD791C</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1006/viro.1995.1115</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-QKMN73G0-P/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001038</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001038</idno><idno type="wicri:Area/Istex/Curation">001038</idno><idno type="wicri:Area/Istex/Checkpoint">001827</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001827</idno><idno type="wicri:doubleKey">0042-6822:1995:Chang C:sequences:in:the</idno><idno type="wicri:Area/Main/Merge">004197</idno><idno type="wicri:Area/Main/Curation">004136</idno><idno type="wicri:Area/Main/Exploration">004136</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation</title><author><name sortKey="Chang, Christopher" sort="Chang, Christopher" uniqKey="Chang C" first="Christopher" last="Chang">Christopher Chang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Howard Hughes Medical Institute and Departments of Microbiology and Immunology, Biochemistry and Medicine, University of California Medical Center, Box 0502, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Hirsch, Russell C" sort="Hirsch, Russell C" uniqKey="Hirsch R" first="Russell C." last="Hirsch">Russell C. Hirsch</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Howard Hughes Medical Institute and Departments of Microbiology and Immunology, Biochemistry and Medicine, University of California Medical Center, Box 0502, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Ganem, Don" sort="Ganem, Don" uniqKey="Ganem D" first="Don" last="Ganem">Don Ganem</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Howard Hughes Medical Institute and Departments of Microbiology and Immunology, Biochemistry and Medicine, University of California Medical Center, Box 0502, San Francisco</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">207</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="549">549</biblScope><biblScope unit="page" to="554">554</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The pregenomic RNA of hepadnaviruses serves as both the mRNA for the core and polymerase proteins and the RNA template for reverse transcription. We have identified a region in the duck hepatitis B virus pregenomic RNA transcription unit that is critical for the accumulation of this transcript. This 85-nt region, termed α, is located within the preC region; deletion of α results in drastically reduced steady-state levels of pregenomic RNA. This effect is not due to reduction in transcription initiation or to enhancement of premature polyadenylation at the 5′ copy of the viral poly(A) signal. However, this phenotype is suppressed by deletion of a second, larger region (β) located ca. 1 kb downstream. The activity of the α element is tissue- and species-nonspecific; however, it displays absolute orientation-dependence and its activity is influenced by its position within the transcript. Models for its action are discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Chang, Christopher" sort="Chang, Christopher" uniqKey="Chang C" first="Christopher" last="Chang">Christopher Chang</name></region><name sortKey="Ganem, Don" sort="Ganem, Don" uniqKey="Ganem D" first="Don" last="Ganem">Don Ganem</name><name sortKey="Hirsch, Russell C" sort="Hirsch, Russell C" uniqKey="Hirsch R" first="Russell C." last="Hirsch">Russell C. Hirsch</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004136 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004136 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:A4ECD776048990F51F3B984A5224A65AB2CD791C
|texte= Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation
}}
| This area was generated with Dilib version V0.6.33. |  |